The development of insect resistance to insecticidal proteins, such as B. thuringiensis (Bt) proteins, is a continuous threat to insect control traits in biotech agricultural crops. This requires constant search for insecticidal proteins with new modes of action (MOA), which enhance insect resistance management options via delaying new resistance development and combating already existing resistant insects. This presentation will describe the application of phage-assisted continuous evolution (PACE) technology to Bt toxins for generating new MOAs, and overcoming Bt resistance in insects.